[Analysis Case] Evaluation of the Molecular Weight Distribution of Hyaluronic Acid
The molecular weight distribution of hyaluronic acid can be evaluated by electrophoresis.
Hyaluronic acid is a high molecular compound widely used in cosmetics and supplements. The characteristics, such as permeability to tissues, vary depending on the molecular weight, and there is a growing demand for low molecular weight hyaluronic acid in addition to high molecular weight hyaluronic acid. In electrophoresis, which is used for the analysis of polysaccharides, DNA, proteins, etc., the molecular weight distribution of hyaluronic acid can be visualized and evaluated by using a hyaluronic acid molecular weight marker with a known molecular weight. Here, we present analytical examples where high molecular weight hyaluronic acid is depolymerized by various methods and its molecular weight distribution is evaluated.
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